By Yangyang Yang
In this publication, the writer bargains typically with subject matters: (1) single-molecule visualization of switching behaviors within the DNA nanoframe process using other kinds of molecular switches by using high-speed atomic strength microscope (AFM); (2) building of photocontrollable DNA nanostructures in programmed styles and direct visualization of the dynamic assembling approach. right here, high-speed AFM was once hired to watch the dynamic routine of unmarried molecules. in comparison to a standard single-molecule research approach, corresponding to fluorescence spectroscopy or electron microscopy, high-speed AFM makes attainable the real-time remark of molecule behaviors. DNA nanostructures have been designed and assembled as scaffolds to include biomolecules. The observations have been conducted less than strong stipulations with out advanced pretreatment. furthermore, the photoresponsive molecules have been effectively assembled into round a hundred nm-sized DNA nanostructures. The assembly/disassembly of nanostructures could be regulated reversibly via photoirradiation. This e-book explains how DNA origami has steadily develop into a useful gizmo for the research of biochemical interactions in outlined nanospace. It additionally exhibits the potential for DNA nanostructures performing as nanodevices for program in organic platforms, serving as an exceptional advent to simple DNA nanotechnology.
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Artificially Controllable Nanodevices Constructed by DNA Origami Technology: Photofunctionalization and Single-Molecule Analysis (Springer Theses) by Yangyang Yang